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KMID : 0606920080160020095
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Biomolecules & Therapeutics
2008 Volume.16 No. 2 p.95 ~ p.99
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Fumonisin B1 Induces Apoptosis in Sphingosine 1-Phosphate Lyase-null F9 Cells through Increase of Sphingolipids Levels
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Pak Seon-Mi
Yoo Hwan-Soo
Lee Yong-Moon
Oh Sei-Kwan
Kim Wan-Jong
Park Nam-Young
Park Myung-Yong
Lee Jong-Hwa
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Abstract
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Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.
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KEYWORD
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Apoptosis, Sphingosine 1-phosphate, Release, F9 cells, HPLC
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